ICR Instruments & Tools
14.5 T / 104 mm Bore System
The flagship of the National High Field FT-ICR Mass Spectrometry Facility, this actively-shielded system is one of the highest-field superconducting ICR magnets in the world. The spectrometer features an electrospray ion source; linear quadrupole trap for external ion storage, mass selection, and collisional dissociation (CAD); and automatic gain control (AGC) for accurate and precise control of charge delivered to the ICR cell. The combination of AGC and high magnetic field makes sub-ppm mass accuracy routine without the need for an internal calibrant. Mass resolving power > 200,000 at m/z 400 is achieved at one scan per second, which is ideal for LC-MS. Robotic sample handling allows unattended or remote operation. An additional pumping stage (for ultrahigh resolution of small molecules) and simultaneous infrared multiphoton (IRMPD) and electron capture dissociation (ECD) are under development.
The 14.5 T, 104 mm bore system.
9.4 T / 220 mm Bore System
This system offers a unique combination of mass resolving power (m/Δm = 8,000,000 at mass 9,000 Da) and dynamic range (>10,000:1), as well as high mass range, mass accuracy, dual-electrospray source for accurate internal mass calibration, efficient tandem mass spectrometry (as high as MS8), and long ion storage period. The magnet is passively shielded to allow proper function of all equipment and safety for users. The system features external mass selection prior to ion injection for further increase in dynamic range and rapid (~100 ms timescale) MS/MS (Anal. Chem., 75, 3256-3262 (2003). Available dissociation techniques include collisional (CAD), photon-induced (Infrared Multiphoton Dissociation (IRMPD), and electron-induced (ECD). A robotic sample-handling system allows unattended and geographically remote operation. An atmospheric pressure photoionization (APPI) source can be used for analysis of nonpolar analytes. HPLC and CE interfaces are also available.
9.4 T and 7 T Actively Shielded Instruments
These FT-ICR instruments are available for analysis of complex nonpolar mixtures. The 9.4 T magnet is currently used for field desorption (Anal. Chem., 77, 1317-1324 (2005)) and matrix-assisted laser desorption/ionization (MALDI). The 7 T magnet is optimized for volatile mixture analysis (Rev. Sci. Instrum., 77, 0000 (2006)). Samples are volatilized in a heated glass inlet system (at 200-300 °C) and externally ionized by an electron beam (0-100 eV, 0.1-10 μA). The ions are collected in a linear multipole ion trap and injected into the FT-ICR cell. Mass resolving power (m/Δm) greater than 105 and mass accuracy within 1 ppm have been achieved with both systems. Thousands of components in a complex mixture (e.g., petroleum distillates) can thus be resolved and identified.
21 T System
A new 21 T state-of-the-art superconducting system is in the development phase.
For more information contact ICR program director Dr. Alan Marshall at marshall@magnet.fsu.edu or (850) 644-0529.
